The DNA helicase UvrD (helicase II) protein plays an important role in nucleotide excision repair, mismatch repair, rolling circular plasmid replication,

Strongly sensitive to UV, ciprofloxacin (CFX), and azidothymidine (AZT) in single deletion mutants, radA-uvrD double deletions are more sensitive yet. Adding recF mutations almost completely suppresses AZT and partially suppresses UV and CFX sensitivity, suggesting RadA processes a class of intermediates that accumulate in uvrD mutants (PubMed: 25484163 ). 1 Publication

UvrD-like DNA helicases unwind DNA with a 3'-5' polarity [ PUBMED:10679457]. Crystal structures of several uvrD-like DNA helicases have been solved [ PUBMED:9288744, PUBMED:10199404, PUBMED:15538360]. Teams. Q&A for work. Connect and share knowledge within a single location that is structured and easy to search.

Uvrd

UvrD and Rep belong to the SF1 family, which shares 40% amino-acid identity and these are remarkably similar to the PcrA helicase of Gram-positive bacteria. The uvrD gene of E. coli encodes a DNA-dependent ATPase. Nature 298:98-100; Arthur, H.M., P.B. Eastlake 1983. Transcriptional control of the uvrD gene of Escherichia coli. Gene 25:309-316; Easton, A.M., S.R. Kushner 1983.

Escherichia coli UvrD is a superfamily 1 helicase/translocase that functions in DNA repair, replication, and recombination. Although a UvrD monomer can translocate along single-stranded DNA, self-assembly or interaction with an accessory protein is needed to activate its helicase activity in vitro. Our previous studies have We have shown that the uvrD gene product, previously identified in maxicell extracts as a 73 kilodalton protein, copurifies with single stranded DNA-dependent ATPase and ATP-dependent DNA helicase activities.

Jan 19, 1993 DNA-Unwinding Dynamics of Escherichia coli UvrD Lacking the C-Terminal 40 Amino Acids. Biophysical Journal 2020, 118 (7) , 1634-1648.

The Rep family helicases are composed of four structural domains. The Rep family function as dimers. The DNA helicase UvrD (helicase II) protein plays an important role in nucleotide excision repair, mismatch repair, rolling circular plasmid replication, Feb 22, 2021 Accessory replicative helicases in Escherichia coli, Rep and UvrD, help replication machinery overcome blocks by removing incoming Mar 30, 2015 The Escherichia coli UvrD protein is a superfamily 1 (SF1) DNA helicase/ translocase that functions in methyl-directed mismatch repair (MMR) (1,2) Buy uvrD recombinant protein, DNA helicase II (uvrD) Recombinant Protein- NP_418258.1 (MBS1216251) product datasheet at MyBioSource, Recombinant A helicase with DNA-dependent ATPase activity (PubMed:8419285).

Escherichia coli UvrD is a superfamily 1 DNA helicase and single-stranded DNA (ssDNA) translocase that functions in DNA repair and plasmid replication and as an anti-recombinase by removing RecA protein from ssDNA. UvrD couples ATP binding and hydrolysis to unwind double-stranded DNA and translocate along ssDNA with 3'-to-5' directionality.

If MutL functions to load UvrD on the DNA, this provides a mechanism to load UvrD exclusively on the appropriate strand. 2008-03-15 · UvrD from E. coli is a 73-kDa protein and was characterized as DNA helicase II (Kumura and Sekiguchi, 1984).

Suzuko Mimori · Song · 2021. Dec 29, 2006 UvrD Helicase Unwinds DNA One Base Pair at a Time by a Two-Part Power Stroke · Summary. Helicases use the energy derived from nucleoside Synonyms, uvr502; srjC; uvrE; dar-2; dda; mutU; pdeB; rad; recL. Accession IDs, EG11064 (EcoCyc) b3813. ECK3808 P03018 (UniProt), Length, 2163 bp / 720 Oct 15, 2013 UvrD is a DNA helicase involved in several DNA repair pathways.
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UvrD monomers can translocate along single-stranded DNA, but self-assembly or interaction with an accessory factor is required to activate processive DNA unwinding in vitro.

Adding recF mutations almost completely suppresses AZT and partially suppresses UV and CFX sensitivity, suggesting RadA processes a class of intermediates that accumulate in uvrD mutants (PubMed UvrD may refer to: UvrABC endonuclease, an enzyme DNA helicase, an enzyme class ‹ The template below (Disambiguation) is being considered for merging. Tte-UvrD was used to develop athermophilichelicase-dependent amplification (tHDA) system to selectively amplify target sequences at 60-65 degrees C. The tHDA system is more efficient than mHDA, displaying heightened amplification sensitivity without the need for the MutL and SSB accessory proteins. Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.
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2020-10-04 · We have recently determined the structure of an important carboxy-terminal domain of the helicase UvrD involved in TCR revealing its role as a binding hub (Kawale & Burmann, Commun. Biol. 2020). Currently, we are focusing on different domains of UvrD to elucidate its functional details. Integrated Structural Biology Approaches

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Status. Unreviewed - Annotation score: Annotation score:2 out of 5. The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.

UvrD-like DNA helicases unwind DNA with a 3'-5' polarity . Crystal structures of several uvrD-like DNA helicases have been solved (see for example ) [4,5,6]. The ONLY lens hood that's easy to pack, install & use. Fits 99% of lenses. Holds ANY size circular filter. Completely removes reflections and glares when shooting through windows & actually enhances workflow. 2012-05-09 · UvrD monomer binding to ssDNA occurs randomly, so after the UvrD:ssDNA complex formed, it was mixed with buffer T 20, ATP, MgCl 2, and heparin.

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Tte UvrD Helicase: M1202: Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. Tth Argonaute: M0665 Moreover, the two distinct activities correlate with the number of UvrD helicases present on the DNA hairpin, measured by counting singly labeled UvrD . When we observed frustrated unwinding activity, we usually detected only a single fluorophore, indicating a single UvrD loaded ( Fig. 2A , top panel). As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

8,194 likes · 53 talking about this. The UNIVERSAL LENS HOOD is LIVE. Click on the link. Pledge NOW & claim one of the last remaining Early Bird Specials while they're still available. You 2020-10-04 · We have recently determined the structure of an important carboxy-terminal domain of the helicase UvrD involved in TCR revealing its role as a binding hub (Kawale & Burmann, Commun. Biol.